Difference between revisions of "Part:BBa K539742:Experience"
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'''Expression analysis in different host cell''' | '''Expression analysis in different host cell''' | ||
| − | We transform the circuit [ | + | We transform the circuit [https://parts.igem.org/Part:BBa_K539742 BBa_K539742] ,which is inserted in pSB4A5, into DH5α and EPI300 and collect the bacteria incubated for 0HR, 24HR and 40 HR to find out the most appropriate host cell. After the analysis of GC we find out that DH5α is much better than EPI300 for this circuit, because it can provide higher productive rate of isobutanol. Therefore, we chose DH5α as the host in the following experiments. |
https://static.igem.org/mediawiki/2011/9/9d/Butanol-10.1.png | https://static.igem.org/mediawiki/2011/9/9d/Butanol-10.1.png | ||
Revision as of 17:06, 5 October 2011
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Applications of BBa_K539742
Experience by 2011 iGEM NCTU_FORMOSA
Expression analysis in different host cell
We transform the circuit BBa_K539742 ,which is inserted in pSB4A5, into DH5α and EPI300 and collect the bacteria incubated for 0HR, 24HR and 40 HR to find out the most appropriate host cell. After the analysis of GC we find out that DH5α is much better than EPI300 for this circuit, because it can provide higher productive rate of isobutanol. Therefore, we chose DH5α as the host in the following experiments.
Figure 10. This diagram indicates that DH5α is very suitable for this circuit Part:BBa_K539742 , because it can provide higher productive rate of isobutanol.
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