Difference between revisions of "Part:BBa K533006"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K533006 short</partinfo> | <partinfo>BBa_K533006 short</partinfo> | ||
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mCherry protein with a his tag for purification and a proline rich peptide for association with SH3 domain. | mCherry protein with a his tag for purification and a proline rich peptide for association with SH3 domain. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | After Pro-rich mCherry expression, significant color change can be observed (bacteria cells turned cherry red as a result of mCherry expression) | ||
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| + | [[Image:Thuexp_cherryexp.png]] | ||
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| + | ''From left to right, IPTG concentration: 0, 0.1mM, 0.5mM, 1mM. All induced at 18 centigrade.'' | ||
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Latest revision as of 16:16, 5 October 2011
Multi-Proline-mCherry
mCherry protein with a his tag for purification and a proline rich peptide for association with SH3 domain.
Usage and Biology
After Pro-rich mCherry expression, significant color change can be observed (bacteria cells turned cherry red as a result of mCherry expression)
From left to right, IPTG concentration: 0, 0.1mM, 0.5mM, 1mM. All induced at 18 centigrade.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 109
Illegal BamHI site found at 908
Illegal XhoI site found at 113 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]