Difference between revisions of "Part:BBa K581011"
 
(3 intermediate revisions by the same user not shown)
Line 17: Line 17:
 
<partinfo>BBa_K581011 parameters</partinfo>
 
<partinfo>BBa_K581011 parameters</partinfo>
 
<!-- -->
 
<!-- -->
−
 
  
 
===Design Notes===
 
===Design Notes===
  
−
▪ A silent mutation (G-A) at base 1116 was made to remove PstI site
+
▪ A silent mutation (G->A) at base 1116 was made to remove XbaI site
−
▪ cqsA was amplified by PCR
+
 
 +
▪ cqsA was amplified by PCR from the genome DNA of ''Vibrio cholera''
  
  
 
===Source===
 
===Source===
  
−
▪ We received the genome DNA of Vibrio cholera  from Chinese Center for Disease Control and Prevention
+
▪ We received the genome DNA of ''Vibrio cholera'' from Chinese Center for Disease Control and Prevention
  
−
▪ Although our system includes several genes from Vibrio cholera, these genes are not directly involved with virulence or antibiotic resistance in any case.
+
▪ Although our system includes several genes from ''Vibrio cholera'', these genes are not directly involved with virulence or antibiotic resistance in any case.

Latest revision as of 16:13, 5 October 2011

pBAD-rbs-cqsA-terminator

A generator of CAI-1 behind pBAD promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 708
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

▪ A silent mutation (G->A) at base 1116 was made to remove XbaI site

▪ cqsA was amplified by PCR from the genome DNA of Vibrio cholera


Source

▪ We received the genome DNA of Vibrio cholera from Chinese Center for Disease Control and Prevention

▪ Although our system includes several genes from Vibrio cholera, these genes are not directly involved with virulence or antibiotic resistance in any case.