Difference between revisions of "Part:BBa K533009:Experience"
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This part is constructed as a tool to instruct E. coli chemotaxis toward a gradient of aspartate. Since it is originally one of the chemotaxis receptors of E. coli, the effect of chemotaxis cannot be seen until all the other chemoreceptors have been knocked out. | This part is constructed as a tool to instruct E. coli chemotaxis toward a gradient of aspartate. Since it is originally one of the chemotaxis receptors of E. coli, the effect of chemotaxis cannot be seen until all the other chemoreceptors have been knocked out. | ||
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| + | Dr. Mark Goulian's lab has characterized the chemotaxis property of this expression cassette in E. coli strain UU1250 (Δtsr-7028 Δ(tar-tap)5201 Δtrg-100 Δaer-1). | ||
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| + | [[Image:mgoulian_tar.jpg]] | ||
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| + | pPD10, the empty vector demonstrated no significant chemotaxis while both pPD12 and pMG65 exhibited chemotaxis towards aspartate gradient. In pMG65, the tar receptor is expressed from a constitutive strong trc promoter while in pPD12 tar is expressed from a very weak promoter inherent in lacI coding sequence. The results indicates that weaker expression of the chemoreceptor makes more sensitive chemotaxis (Derr, Boder et al. 2006). | ||
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| + | ====Reference==== | ||
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| + | ''Derr, P., E. Boder, et al. (2006). "Changing the specificity of a bacterial chemoreceptor." J Mol Biol 355(5): 923-932.'' | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 15:57, 5 October 2011
Applications of BBa_K533009
This part is constructed as a tool to instruct E. coli chemotaxis toward a gradient of aspartate. Since it is originally one of the chemotaxis receptors of E. coli, the effect of chemotaxis cannot be seen until all the other chemoreceptors have been knocked out.
Dr. Mark Goulian's lab has characterized the chemotaxis property of this expression cassette in E. coli strain UU1250 (Δtsr-7028 Δ(tar-tap)5201 Δtrg-100 Δaer-1).
pPD10, the empty vector demonstrated no significant chemotaxis while both pPD12 and pMG65 exhibited chemotaxis towards aspartate gradient. In pMG65, the tar receptor is expressed from a constitutive strong trc promoter while in pPD12 tar is expressed from a very weak promoter inherent in lacI coding sequence. The results indicates that weaker expression of the chemoreceptor makes more sensitive chemotaxis (Derr, Boder et al. 2006).
Reference
Derr, P., E. Boder, et al. (2006). "Changing the specificity of a bacterial chemoreceptor." J Mol Biol 355(5): 923-932.
User Reviews
UNIQ6696393b019a4fd7-partinfo-00000000-QINU UNIQ6696393b019a4fd7-partinfo-00000001-QINU