Difference between revisions of "Part:BBa K524004"
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<partinfo>BBa_K524004 short</partinfo> | <partinfo>BBa_K524004 short</partinfo> | ||
− | The pir gene | + | The pir gene encodes the π protein. Initiation of replication at ori-γ or other R6K origins of replication requires the π protein in trans. Increasing its expression to double the natural concentration produced would, however, effectively shut down the replication. Expression of π protein is autogenously regulated. |
===Usage and Biology=== | ===Usage and Biology=== | ||
− | The presence of pir gene | + | The presence of pir gene allow maintenance of plasmids with R6K origins as their sole origin of replication. |
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Latest revision as of 15:48, 5 October 2011
pir gene
The pir gene encodes the π protein. Initiation of replication at ori-γ or other R6K origins of replication requires the π protein in trans. Increasing its expression to double the natural concentration produced would, however, effectively shut down the replication. Expression of π protein is autogenously regulated.
Usage and Biology
The presence of pir gene allow maintenance of plasmids with R6K origins as their sole origin of replication.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 438
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]