Difference between revisions of "Part:BBa K524003:Design"
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===Design Notes===
 
===Design Notes===
  
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The <i>nadE</i> gene in <i>E. Coli</i> chromosome is terminated by terminated by downstream extragenic sites. The extragenic sties were replaced with double terminator B0015 in our construct.
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The <i>nadE</i> gene in the <i>E. coli</i> genome is originally terminated by downstream extragenic sites. The extragenic sties were replaced with the double terminator [https://parts.igem.org/Part:BBa_B0015 BBa_B0015] in our construct.
  
 
===Source===
 
===Source===

Latest revision as of 15:43, 5 October 2011

nadE gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The nadE gene in the E. coli genome is originally terminated by downstream extragenic sites. The extragenic sties were replaced with the double terminator BBa_B0015 in our construct.

Source

The nadE gene was cloned out from the genome of E. coli strain BL21(DE3).

References

Ecocyc: Encyclopedia of Escherichia coli K-12 Genes and Metabolism