Difference between revisions of "Part:BBa K629002"

Revision as of 15:41, 5 October 2011

recNp, could be started with exposure to irradiation, UV, nalidixic acid

Escherichia coli cells which contain a functional Kup (formerly TrkD) system took up Cs' with a moderate rate and affinity. Kup is a separate K+ uptake system with relatively little discrimination in the transport of the cations K+, Rb+, and Cs'. Regardless of the presence or absence of Kup, K+-replete cells took up Cs' primarily by a very low affinity mode, proportional to the ratio of the Cs' and K+ concentrations in the medium.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K629002 short</partinfo>
-Name:	recNp
+Escherichia coli cells which contain a functional Kup (formerly TrkD) system took up Cs' with a moderate rate and affinity. Kup is a separate K+ uptake system with relatively little discrimination in the transport of the cations K+, Rb+, and Cs'. Regardless of the presence or absence of Kup, K+-replete cells took up Cs' primarily by a very low affinity mode, proportional to the ratio of the Cs' and K+ concentrations in the medium.
-+1:	2749781
-Sigma Factor:	Sigma70 Sigmulon
-Distance from start of the gene:	36
-Sequence:	 aattattctaattttacgccagcctctttactgtatataaaaccagtttatactgtacacAataacagtaatggtttttca
-                        -35                   -10          +1
-Note(s):	We assigned a putative transcription start site to this promoter based on the observation that the majority of the promoters, whose transcription start sites were determined experimentally, present a distance of 6 nucleotides between the transcription start site and the -10 box.
-Evidence(s):	 [HIPP] Human inference of promoter position
-Reference(s):	[1] Rostas K., et al., 1987
 <!-- Add more about the biology of this part here