Difference between revisions of "Part:BBa K533005:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | His tag is inserted between OmpA and GFP for easier detection in Western Blotting. | + | T7 promoter driven OmpA-GFP fusion protein. His tag is inserted between OmpA and GFP for easier detection in Western Blotting. |
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===Source=== | ===Source=== | ||
Latest revision as of 15:28, 5 October 2011
OmpA-GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1419
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
T7 promoter driven OmpA-GFP fusion protein. His tag is inserted between OmpA and GFP for easier detection in Western Blotting.
Source
OmpA is cloned from BL21(DE3) E. Coli strain. GFP is from Clontech plasmid pGFP-N1