Difference between revisions of "Part:BBa K624033"
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This part, minC, is known as a cell division inhibitor. Its first record on iGEM was made by 2006 Chiba iGEM team([https://parts.igem.org/partsdb/get_part.cgi?part=BBa_J29040 BBa_J29040]), but there is not an available part. 2010 Warsaw iGEM team submitted another minC ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K299806 BBa_K299806]) with different sequence. However, there was a PstI site in the middle of the sequence.  
 
This part, minC, is known as a cell division inhibitor. Its first record on iGEM was made by 2006 Chiba iGEM team([https://parts.igem.org/partsdb/get_part.cgi?part=BBa_J29040 BBa_J29040]), but there is not an available part. 2010 Warsaw iGEM team submitted another minC ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K299806 BBa_K299806]) with different sequence. However, there was a PstI site in the middle of the sequence.  
 +
 
We designed primers to remove PstI the site with a synonymous substitution. This makes it easier to do EXSP biobrick assembly.
 
We designed primers to remove PstI the site with a synonymous substitution. This makes it easier to do EXSP biobrick assembly.
  

Revision as of 15:12, 5 October 2011

minC cell division inhibitor (revised)

This part, minC, is known as a cell division inhibitor. Its first record on iGEM was made by 2006 Chiba iGEM team(BBa_J29040), but there is not an available part. 2010 Warsaw iGEM team submitted another minC (BBa_K299806) with different sequence. However, there was a PstI site in the middle of the sequence.

We designed primers to remove PstI the site with a synonymous substitution. This makes it easier to do EXSP biobrick assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 211
  • 1000
    COMPATIBLE WITH RFC[1000]