Difference between revisions of "Part:BBa K624033"
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| − | This part, minC, is known as a cell division inhibitor. It was first submitted by 2010 Warsaw iGEM team.(https://parts.igem.org/wiki/index.php?title=Part:BBa_K299806) However, there was a PstI site in the middle of the sequence, which makes it hard to do biobrick assembly. We designed primers to remove the site with a synonymous substitution. | + | This part, minC, is known as a cell division inhibitor. It was first submitted by 2010 Warsaw iGEM team.([https://parts.igem.org/wiki/index.php?title=Part:BBa_K299806 BBa_K299806]) However, there was a PstI site in the middle of the sequence, which makes it hard to do biobrick assembly. We designed primers to remove the site with a synonymous substitution. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 14:41, 5 October 2011
minC cell division inhibitor (revised)
This part, minC, is known as a cell division inhibitor. It was first submitted by 2010 Warsaw iGEM team.(BBa_K299806) However, there was a PstI site in the middle of the sequence, which makes it hard to do biobrick assembly. We designed primers to remove the site with a synonymous substitution.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 211
- 1000COMPATIBLE WITH RFC[1000]