Difference between revisions of "Part:BBa K629001:Design"

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(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
−
the -35 and -10 district, the RBS
+
contains the original RBS after the promoter
−
 
+
−
 
+
  
 
===Source===
 
===Source===
  
−
E. Coli BL21 plys genome
+
genome of E. Coli BL21 plys
  
 
===References===
 
===References===
−
Shana Topp and Justin P. Gallivan,Guiding Bacteria with Small Molecules and RNA, JACS, 2007, 129, 27, 6807-6811
+
S. NUYTS. et al. Insertion or Deletion of the Cheo Box Modifies Radiation Inducibility
 +
of Clostridium Promoters. ''APPLIED AND ENVIRONMENTAL MICROBIOLOGY'' '''67'''(10). 4464-4470(2001).
 +
 
 +
S Nuyts. et al. Radio-responsive recA promoter significantly increases TNFa production in recombinant clostridia after 2 Gy irradiation. ''Gene Therapy'' '''8'''. 1197-1201(2001).
 +
 
 +
Sandra Nuyts. et al. The Use of  Radiation-Induced Bacterial Promoters in Anaerobic Conditions: A Means to Control Gene Expression in Clostridium-Mediated Therapy for Cancer. ''RADIATION  RESEARCH'' '''155'''. 716~723(2001).

Latest revision as of 14:18, 5 October 2011

recAp, could be started with exposure to irradiation, UV, nalidixic acid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

contains the original RBS after the promoter

Source

genome of E. Coli BL21 plys

References

S. NUYTS. et al. Insertion or Deletion of the Cheo Box Modifies Radiation Inducibility of Clostridium Promoters. APPLIED AND ENVIRONMENTAL MICROBIOLOGY 67(10). 4464-4470(2001).

S Nuyts. et al. Radio-responsive recA promoter significantly increases TNFa production in recombinant clostridia after 2 Gy irradiation. Gene Therapy 8. 1197-1201(2001).

Sandra Nuyts. et al. The Use of Radiation-Induced Bacterial Promoters in Anaerobic Conditions: A Means to Control Gene Expression in Clostridium-Mediated Therapy for Cancer. RADIATION RESEARCH 155. 716~723(2001).