Difference between revisions of "Part:BBa K533003:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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===Applications of BBa_K533003===
 
===Applications of BBa_K533003===
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Like [[Part:BBa_I712667 |  BBa_I712667]], it can specifically cleave proteins in the peptide. The difference is that it is expressed as a membrane protein, which enables it to cut target proteins in the media.
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We tested function of the protease with OmpA-HIV protese site-SH3, our part [[Part:BBa_K533002 | BBa_K533002]].
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E. coli expressing OmpA-HIV protease site-SH3 were first mixed up in multi-proline mCherry, to give it the red fluorescence marker. After washing with distilled water twice, the remaining bacteria were mixed with E. coli expressing OmpA-HIV protease at room temperature for 30 minutes to release the cargo.
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After washing with distilled water twice, bacteria were fixed on a slip and stained  with DAPI.
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{| style="background: none; text-align:center;" align="center"
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| Color Channel
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| + E. coli expressing protease
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| + E. coli without protease
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|-
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| mCherry
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| [[Image:Thuexp_release_p.png|350px]]
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| [[Image:Thuexp_release_n.png|350px]]
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|-
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| DAPI
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| [[Image:Thuexp_release_p_d.png|350px]]
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| [[Image:Thuexp_release_n_d.png|350px]]
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|-
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| merge
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| [[Image:Thuexp_release_p_m.png|350px]]
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| [[Image:Thuexp_release_n_m.png|350px]]
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|}
  
 
===User Reviews===
 
===User Reviews===

Revision as of 14:04, 5 October 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K533003

Like BBa_I712667, it can specifically cleave proteins in the peptide. The difference is that it is expressed as a membrane protein, which enables it to cut target proteins in the media.

We tested function of the protease with OmpA-HIV protese site-SH3, our part BBa_K533002.

E. coli expressing OmpA-HIV protease site-SH3 were first mixed up in multi-proline mCherry, to give it the red fluorescence marker. After washing with distilled water twice, the remaining bacteria were mixed with E. coli expressing OmpA-HIV protease at room temperature for 30 minutes to release the cargo.

After washing with distilled water twice, bacteria were fixed on a slip and stained with DAPI.


Color Channel + E. coli expressing protease + E. coli without protease
mCherry Thuexp release p.png Thuexp release n.png
DAPI Thuexp release p d.png Thuexp release n d.png
merge Thuexp release p m.png Thuexp release n m.png

User Reviews

UNIQ0ae22ce130839bfc-partinfo-00000000-QINU UNIQ0ae22ce130839bfc-partinfo-00000001-QINU