Difference between revisions of "Part:BBa K524100:Design"

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'GUG' start codon has been mutated to 'AUG'. Stop codons at the end of the coding sequence have been changed to 'TAATAA'.
 
'GUG' start codon has been mutated to 'AUG'. Stop codons at the end of the coding sequence have been changed to 'TAATAA'.
  
−
The SpeI site at the suffix end was deleted for unknown reasons. However, this should not affect the functionality of the protein.
+
The double terminator (BBa_B0015) has been attached via non-standard assembly methods.
  
 
===Source===
 
===Source===
Line 15: Line 15:
  
 
===References===
 
===References===
−
Ecocyc: Encyclopedia of Escherichia coli K-12 Genes and Metabolism [http://ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG11419]
+
Ecocyc: Encyclopedia of <i>Escherichia coli</i> K-12 Genes and Metabolism [http://ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG11419]
  
 
Kunihiko Nishino, Eiji Nikaido, Akihito Yamaguchi, Regulation and physiological function of multidrug efflux pumps in Escherichia coli and Salmonella, Biochimica et Biophysica Acta (BBA) - Proteins &amp; Proteomics, Volume 1794, Issue 5, May 2009, Pages 834-843, ISSN 1570-9639, 10.1016/j.bbapap.2009.02.002.
 
Kunihiko Nishino, Eiji Nikaido, Akihito Yamaguchi, Regulation and physiological function of multidrug efflux pumps in Escherichia coli and Salmonella, Biochimica et Biophysica Acta (BBA) - Proteins &amp; Proteomics, Volume 1794, Issue 5, May 2009, Pages 834-843, ISSN 1570-9639, 10.1016/j.bbapap.2009.02.002.
 
[http://www.sciencedirect.com/science/article/pii/S1570963909000260]
 
[http://www.sciencedirect.com/science/article/pii/S1570963909000260]

Latest revision as of 14:02, 5 October 2011

Bcr - multidrug efflux system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 503
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 99
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

'GUG' start codon has been mutated to 'AUG'. Stop codons at the end of the coding sequence have been changed to 'TAATAA'.

The double terminator (BBa_B0015) has been attached via non-standard assembly methods.

Source

Amplified from genomic DNA of Escherichia coli K-12 Strain RR1

References

Ecocyc: Encyclopedia of Escherichia coli K-12 Genes and Metabolism [http://ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG11419]

Kunihiko Nishino, Eiji Nikaido, Akihito Yamaguchi, Regulation and physiological function of multidrug efflux pumps in Escherichia coli and Salmonella, Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics, Volume 1794, Issue 5, May 2009, Pages 834-843, ISSN 1570-9639, 10.1016/j.bbapap.2009.02.002. [http://www.sciencedirect.com/science/article/pii/S1570963909000260]