Difference between revisions of "Part:BBa K524100:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K524100 short</partinfo>
 
<partinfo>BBa_K524100 short</partinfo>
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===Design Notes===
 
===Design Notes===
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'GTG' start codon has been mutated to 'ATG'. Stop codons at the end of the coding sequence have been changed to 'TAATAA'.  
+
'GUG' start codon has been mutated to 'AUG'. Stop codons at the end of the coding sequence have been changed to 'TAATAA'.
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+
  
 +
The double terminator (BBa_B0015) has been attached via non-standard assembly methods.
  
 
===Source===
 
===Source===
  
−
Amplified from genomic DNA of Escherichia coli K-12 Strain RR1 via PCR
+
Amplified from genomic DNA of ''Escherichia coli'' K-12 Strain RR1
  
 
===References===
 
===References===
 +
Ecocyc: Encyclopedia of <i>Escherichia coli</i> K-12 Genes and Metabolism [http://ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG11419]
 +
 +
Kunihiko Nishino, Eiji Nikaido, Akihito Yamaguchi, Regulation and physiological function of multidrug efflux pumps in Escherichia coli and Salmonella, Biochimica et Biophysica Acta (BBA) - Proteins &amp; Proteomics, Volume 1794, Issue 5, May 2009, Pages 834-843, ISSN 1570-9639, 10.1016/j.bbapap.2009.02.002.
 +
[http://www.sciencedirect.com/science/article/pii/S1570963909000260]

Latest revision as of 14:02, 5 October 2011

Bcr - multidrug efflux system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 503
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 99
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

'GUG' start codon has been mutated to 'AUG'. Stop codons at the end of the coding sequence have been changed to 'TAATAA'.

The double terminator (BBa_B0015) has been attached via non-standard assembly methods.

Source

Amplified from genomic DNA of Escherichia coli K-12 Strain RR1

References

Ecocyc: Encyclopedia of Escherichia coli K-12 Genes and Metabolism [http://ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG11419]

Kunihiko Nishino, Eiji Nikaido, Akihito Yamaguchi, Regulation and physiological function of multidrug efflux pumps in Escherichia coli and Salmonella, Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics, Volume 1794, Issue 5, May 2009, Pages 834-843, ISSN 1570-9639, 10.1016/j.bbapap.2009.02.002. [http://www.sciencedirect.com/science/article/pii/S1570963909000260]