Difference between revisions of "Part:BBa K524003:Design"
(→‎Design Notes)
Line 7: Line 7:
 
===Design Notes===
 
===Design Notes===
  
−
The <i>nadE<i/> gene in E. Coli chromosome is terminated by terminated by downstream extragenic sites. The extragenic sties were replaced with double terminator B0015 in our construct.
+
The <i>nadE</i> gene in E. Coli chromosome is terminated by terminated by downstream extragenic sites. The extragenic sties were replaced with double terminator B0015 in our construct.
  
 
===Source===
 
===Source===

Revision as of 13:49, 5 October 2011

nadE gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The nadE gene in E. Coli chromosome is terminated by terminated by downstream extragenic sites. The extragenic sties were replaced with double terminator B0015 in our construct.

Source

The nadE gene was cloned out from the genome of E. coli strain BL21(DE3).

References

Ecocyc: Encyclopedia of Escherichia coli K-12 Genes and Metabolism