Difference between revisions of "Part:BBa K524003:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K524003 short</partinfo>
 
<partinfo>BBa_K524003 short</partinfo>
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===Design Notes===
 
===Design Notes===
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nil
 
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The <i>nadE<i/> gene in E. Coli chromosome is terminated by terminated by downstream extragenic sites. The extragenic sties were replaced with double terminator B0015 in our construct.
  
 
===Source===
 
===Source===
  
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From genomic DNA of BL21(DE3)
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The nadE gene was cloned out from the genome of E. coli strain BL21(DE3).
  
 
===References===
 
===References===
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Ecocyc: Encyclopedia of Escherichia coli K-12 Genes and Metabolism

Revision as of 13:49, 5 October 2011

nadE gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The nadE<i/> gene in E. Coli chromosome is terminated by terminated by downstream extragenic sites. The extragenic sties were replaced with double terminator B0015 in our construct.

Source

The nadE gene was cloned out from the genome of E. coli strain BL21(DE3).

References

Ecocyc: Encyclopedia of Escherichia coli K-12 Genes and Metabolism