|
|
| (3 intermediate revisions by the same user not shown) |
| Line 3: |
Line 3: |
| | how you used this part and how it worked out. | | how you used this part and how it worked out. |
| | | | |
| − | ===Construction of BBa_K567015===
| |
| − |
| |
| − | Rational design of MetRS: we have obtained a truncated MetRS without anticodon recognition domain. The structure of the truncated protein is shown below. This modified MetRS can charge tRNA<sup>Met</sup> without recognizing its anticodon.
| |
| − |
| |
| − | [[image:11SJTU-MetRS.jpg|frame|center|fig. This design is based on the crystal structure of methionyl-tRNA synthetase complex with tRNA (PDB ID：2CSX). We have superimposed the crystal structure of methionyl-tRNA synthetase from ''E.coli'' and obtained the overlay structure after kinetics optimization. Above is the picture showing ''E.coli'' methionyl-tRNA synthetase with (left) and without (right) anticodon recognition domain. The picture proposed that ''E.coli'' methionyl-tRNA synthetase will lose the ability to bind tRNA<sup>Met</sup> anticodon if anticodon recognition domain is deleted, thus losing anticodon specificity while maintaining aminoacylation ability. '''We have built the truncated MetRS, PT7-''metG''N (BBa_K567015), based on this design. ''']]
| |
| | | | |
| | ===User Reviews=== | | ===User Reviews=== |
Latest revision as of 10:35, 5 October 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
User Reviews
UNIQ1436b176482c08ab-partinfo-00000000-QINU
UNIQ1436b176482c08ab-partinfo-00000001-QINU
