Difference between revisions of "Part:BBa K524004"
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<partinfo>BBa_K524004 short</partinfo>
 
<partinfo>BBa_K524004 short</partinfo>
  
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The pir gene codes for the pi protein. Initiation of replication at ori-gamma requires the pi protein in trans. Doubling the concentration of pi protein would, however, effectively shut down the replication. Expression of pi protein is autogenously regulated.  
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The pir gene encodes the pi protein. Initiation of replication at ori-gamma or other R6K origin of replication requires the pi protein in trans. Doubling the concentration of pi protein would, however, effectively shut down the replication. Expression of pi protein is autogenously regulated.  
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 10:34, 5 October 2011

pir gene

The pir gene encodes the pi protein. Initiation of replication at ori-gamma or other R6K origin of replication requires the pi protein in trans. Doubling the concentration of pi protein would, however, effectively shut down the replication. Expression of pi protein is autogenously regulated.

Usage and Biology

The presence of pir gene can allow maintenance of plasmids with R6K origins as their sole origin of replication.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 438
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]