Difference between revisions of "Part:BBa K649104"

Revision as of 09:51, 5 October 2011

PlsrA-RBS-gfp We characterized BBa_K649104 and BBa_K117002 in lsrR(-) strain in E.coli.

Fluorescence intensity of BBa_K649104 was much higher than that of promoterless-gfp(negative control).
This work is done by Takuya Tsubaki.

We measured the transcriptional activity of our lsrA promoter(BBa_K649100) by introducing a gfp gene downstream of the promoter. Its fluorescence intensity was much higher than that of promoterless-gfp(negative control), showing that our new lsrA promoter works. Moreover,fluorescence intensity of lsrA promoter-gfp((BBa_K117002)-gfp) was almost the same as a promoterless-gfp(negative control), showing that lsrA promoter(BBa_K117002) does not work properly.

We improved previous lsrA promoter(BBa_K117002).our assay of BBa_K117002

For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#5. our work in Tokyo_Tech 2011 wiki].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 770

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+We characterized BBa_K649104 and BBa_K117002 in lsrR(-) strain in E.coli.
 [[Image:Activity.png|thumb|center|700px|Fluorescence intensity of BBa_K649104 was much higher than that of promoterless-gfp(negative control).<br>This work is done by Takuya Tsubaki.]]