Difference between revisions of "Part:BBa K598018:Design"

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===Design Notes===
 
===Design Notes===
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keep the functional characters of both proteins  
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This part is a translational fusion of TetA and GFPuv linked by a flexible peptide linker. TetA is an inner-membrane protein that consists of 12 transmembrane segments, and its N-terminus and C-terminus are exposed to the cytoplasm. TetA can retain its tetracycline-resistant phenotype when fused to other proteins at the C-terminus. GFPuv has also been fused to membrane proteins in E. coli. A flexible peptide linker sequence encoding (Gly-Gly-Gly-Ser)4 was inserted between TetA and GFPuv to facilitate proper folding of each proteins.
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===Source===
 
===Source===

Revision as of 06:02, 5 October 2011

tetA+GFP fused protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 18
    Illegal BamHI site found at 311
    Illegal BamHI site found at 1786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 337
    Illegal NgoMIV site found at 705
    Illegal NgoMIV site found at 865
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is a translational fusion of TetA and GFPuv linked by a flexible peptide linker. TetA is an inner-membrane protein that consists of 12 transmembrane segments, and its N-terminus and C-terminus are exposed to the cytoplasm. TetA can retain its tetracycline-resistant phenotype when fused to other proteins at the C-terminus. GFPuv has also been fused to membrane proteins in E. coli. A flexible peptide linker sequence encoding (Gly-Gly-Gly-Ser)4 was inserted between TetA and GFPuv to facilitate proper folding of each proteins.

Source

From Yohei Yokobayashi et al. Nucleic Acids Research, 2009, Vol. 37, No. 5

References