Difference between revisions of "Part:BBa K649207:Design"

 
 
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<partinfo>BBa_K649207 short</partinfo>
 
<partinfo>BBa_K649207 short</partinfo>
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===Design Notes===
 
===Design Notes===
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BBa_K649207 is composed of lox2272(BBa_K649204) and PlacIQ-gfp(BBa_J54202). BBa_J54202 contains ''Bgl'' II and ''Mlu'' I restriction site on upstream of gfp. These restriction sites allows insertion of the lox2272,designed to be flanked by the sticky ends of ''BamH'' I and ''Mlu'' I. This lox2272 was inserted to the PlacIQ-gfp digested with ''Bgl'' II and ''Mlu'' I.
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===Source===
 
===Source===
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===References===
 
===References===
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Construction of a genetic AND gate under a new standard for assembly of genetic parts. Ayukawa ''et al''. BMC Genomics. 2010.

Latest revision as of 00:38, 5 October 2011

PlacIQ-lox2272-gfp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 107
    Illegal BamHI site found at 13
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 790


Design Notes

BBa_K649207 is composed of lox2272(BBa_K649204) and PlacIQ-gfp(BBa_J54202). BBa_J54202 contains Bgl II and Mlu I restriction site on upstream of gfp. These restriction sites allows insertion of the lox2272,designed to be flanked by the sticky ends of BamH I and Mlu I. This lox2272 was inserted to the PlacIQ-gfp digested with Bgl II and Mlu I.

Source

References

Construction of a genetic AND gate under a new standard for assembly of genetic parts. Ayukawa et al. BMC Genomics. 2010.