Difference between revisions of "Part:BBa K539392:Design"

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===Design Notes===
 
===Design Notes===
 
In front of this parts ,you can apply to your design.
 
In front of this parts ,you can apply to your design.
 +
 +
We have designed the primer to correct the point mutation of XbaI site already.
 +
 +
Please refer to [https://parts.igem.org/Part:BBa_K274004:Experience BBa_K274004 Experience]
  
 
===Source===
 
===Source===
  
−
vioABDE([[Part:BBa_K274003]]) is taken from 2010 Slovenia iGEM
+
vioABDE([[Part:BBa_K274004]]) is taken from 2008 Cambridge iGEM
  
 
===References===
 
===References===

Latest revision as of 19:02, 4 October 2011

Vio operon ABDE(each preceded by their own ribosome binding site) with a terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1029
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3814
    Illegal NgoMIV site found at 6040
    Illegal AgeI site found at 4010
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 5239
    Illegal SapI.rc site found at 5314


Design Notes

In front of this parts ,you can apply to your design.

We have designed the primer to correct the point mutation of XbaI site already.

Please refer to BBa_K274004 Experience

Source

vioABDE(Part:BBa_K274004) is taken from 2008 Cambridge iGEM

References