Difference between revisions of "Part:BBa K598010:Design"
(→Design Notes) |
(→Source) |
||
| Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
| − | BBa_I13453, BBa_E0040, and BBa_B0015 come from the constitutive | + | BBa_I13453, BBa_E0040, and BBa_B0015 come from the constitutive library. |
RNA controller sequence is inserted between promoter and AUG initiation codon via PCR and bluntend ligation. | RNA controller sequence is inserted between promoter and AUG initiation codon via PCR and bluntend ligation. | ||
===References=== | ===References=== | ||
Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004).A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research 32, 1610-1614. | Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004).A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research 32, 1610-1614. | ||
Latest revision as of 15:26, 4 October 2011
pBAD+Theophylline Responsive Riboswitch 1G1 with Engineered RBS+E0040+B0015
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
Illegal XhoI site found at 209 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 868
Design Notes
This part is designed to test the performance of ligand responsive RNA controller. GFP is used as reporter gene. Change the promoter, terminater, reporter gene, or RNA controller if necessary. Check the RBS sequence before using.
Source
BBa_I13453, BBa_E0040, and BBa_B0015 come from the constitutive library. RNA controller sequence is inserted between promoter and AUG initiation codon via PCR and bluntend ligation.
References
Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004).A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research 32, 1610-1614.