Difference between revisions of "Part:BBa K629006:Design"
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===References=== | ===References=== | ||
− | IRK BOSSEMEYER. et al. Specific Cesium Transport via the Escherichia coli Kup (TrkD) K+ Uptake System.JOURNAL OF BACTERIOLOGY '''171'''(4), 2219-2221(1989). | + | IRK BOSSEMEYER. et al. Specific Cesium Transport via the Escherichia coli Kup (TrkD) K+ Uptake System.''JOURNAL OF BACTERIOLOGY'' '''171'''(4), 2219-2221(1989). |
− | Simon V. Avery. | + | Simon V. Avery. Caesium accumulation by microorganisms: uptake mechanisms, cation competition, compartmentalization and toxicity. ''Journal of Industrial Microbiology'' '''14'''. 76-84(1995). |
− | JANE E. LOPILATO. et al.D-Ribose Metabolism in Escherichia coli K-12: Genetics, Regulation, and Transport. JOURNAL OF BACTERIOLOGY '''158'''(2), 665-673(1984). | + | JANE E. LOPILATO. et al.D-Ribose Metabolism in Escherichia coli K-12: Genetics, Regulation, and Transport. ''JOURNAL OF BACTERIOLOGY'' '''158'''(2), 665-673(1984). |
− | DONALD C. DOSCH.et al. Genetic Analysis of Potassium Transport Loci in Escherichia coli: Evidence for Three Constitutive Systems Mediating Uptake of Potassium. JOURNAL OF BACTERIOLOGY '''173'''(2). 687-696(1991). | + | DONALD C. DOSCH.et al. Genetic Analysis of Potassium Transport Loci in Escherichia coli: Evidence for Three Constitutive Systems Mediating Uptake of Potassium. ''JOURNAL OF BACTERIOLOGY'' '''173'''(2). 687-696(1991). |
− | Joy Perkins | + | Joy Perkins and Geoffrey M.Gadd. The influence of pH and external K+ concentration on caesium toxicity and accumulation in Escherichia coli and Bacillus subtilis. ''Journal of Industrial Microbiology'' '''14'''. 219-225(1995). |
Revision as of 13:35, 4 October 2011
trkD', part of recNp-trkD-egfp, without terminator
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 245
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1320
Design Notes
without terminator
Source
ATGAGCACTGATAATAAGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGAGTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGTCCGGCCAGTTTGGTTTTGGCGTTGAACGCGATGCCGTGTTTGGCTTTTTATCGCTGATCTTCTGGCTGCTAATCTTTGTGGTTTCCATTAAATATCTCACCTTCGTGATGCGGGCAGATAACGCCGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAGCATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCGATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGCTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGACACTTTGGTAAGTTCCCTATTCGTCTGGCGTGGTTCACTGTCGTATTACCTTCCTTAACTCTTAATTACTTCGGCCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTTTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATCGCCTCGCAGGCGGTTATCTCTGGCGTCTTTTCATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATC
References
IRK BOSSEMEYER. et al. Specific Cesium Transport via the Escherichia coli Kup (TrkD) K+ Uptake System.JOURNAL OF BACTERIOLOGY 171(4), 2219-2221(1989).
Simon V. Avery. Caesium accumulation by microorganisms: uptake mechanisms, cation competition, compartmentalization and toxicity. Journal of Industrial Microbiology 14. 76-84(1995).
JANE E. LOPILATO. et al.D-Ribose Metabolism in Escherichia coli K-12: Genetics, Regulation, and Transport. JOURNAL OF BACTERIOLOGY 158(2), 665-673(1984).
DONALD C. DOSCH.et al. Genetic Analysis of Potassium Transport Loci in Escherichia coli: Evidence for Three Constitutive Systems Mediating Uptake of Potassium. JOURNAL OF BACTERIOLOGY 173(2). 687-696(1991).
Joy Perkins and Geoffrey M.Gadd. The influence of pH and external K+ concentration on caesium toxicity and accumulation in Escherichia coli and Bacillus subtilis. Journal of Industrial Microbiology 14. 219-225(1995).