Difference between revisions of "Part:BBa K629006:Design"

 
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<partinfo>BBa_K629006 short</partinfo>
 
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===References===
 
===References===
 +
IRK BOSSEMEYER. et al. Specific Cesium Transport via the Escherichia coli Kup (TrkD) K+ Uptake System.JOURNAL OF BACTERIOLOGY '''171'''(4), 2219-2221(1989).
 +
 +
Simon V. Avery.  Caesium accumulation by microorganisms: uptake mechanisms, cation competition, compartmentalization and toxicity. Journal  of Industrial Microbiology '''14'''. 76-84(1995).
 +
 +
JANE E. LOPILATO. et al.D-Ribose Metabolism in Escherichia coli K-12: Genetics, Regulation, and Transport. JOURNAL OF BACTERIOLOGY '''158'''(2), 665-673(1984).
 +
 +
DONALD C. DOSCH.et al. Genetic Analysis of Potassium Transport Loci in Escherichia coli: Evidence for Three Constitutive Systems Mediating Uptake of Potassium. JOURNAL OF BACTERIOLOGY '''173'''(2). 687-696(1991).
 +
 +
Joy Perkins  and Geoffrey M.  Gadd. The  influence  of  pH  and  external  K  §  concentration  on  caesium  toxicity and  accumulation  in  Escherichia  coli  and  Bacillus  subtilis. Journal  of  Industrial Microbiology '''14'''. 219-225(1995).

Revision as of 13:28, 4 October 2011

trkD', part of recNp-trkD-egfp, without terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 245
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1320


Design Notes

without terminator


Source

ATGAGCACTGATAATAAGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGAGTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGTCCGGCCAGTTTGGTTTTGGCGTTGAACGCGATGCCGTGTTTGGCTTTTTATCGCTGATCTTCTGGCTGCTAATCTTTGTGGTTTCCATTAAATATCTCACCTTCGTGATGCGGGCAGATAACGCCGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAGCATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCGATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGCTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGACACTTTGGTAAGTTCCCTATTCGTCTGGCGTGGTTCACTGTCGTATTACCTTCCTTAACTCTTAATTACTTCGGCCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTTTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATCGCCTCGCAGGCGGTTATCTCTGGCGTCTTTTCATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATC

References

IRK BOSSEMEYER. et al. Specific Cesium Transport via the Escherichia coli Kup (TrkD) K+ Uptake System.JOURNAL OF BACTERIOLOGY 171(4), 2219-2221(1989).

Simon V. Avery. Caesium accumulation by microorganisms: uptake mechanisms, cation competition, compartmentalization and toxicity. Journal of Industrial Microbiology 14. 76-84(1995).

JANE E. LOPILATO. et al.D-Ribose Metabolism in Escherichia coli K-12: Genetics, Regulation, and Transport. JOURNAL OF BACTERIOLOGY 158(2), 665-673(1984).

DONALD C. DOSCH.et al. Genetic Analysis of Potassium Transport Loci in Escherichia coli: Evidence for Three Constitutive Systems Mediating Uptake of Potassium. JOURNAL OF BACTERIOLOGY 173(2). 687-696(1991).

Joy Perkins and Geoffrey M. Gadd. The influence of pH and external K § concentration on caesium toxicity and accumulation in Escherichia coli and Bacillus subtilis. Journal of Industrial Microbiology 14. 219-225(1995).