Difference between revisions of "Part:BBa K649301"
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In our test, when this part was ligated into pSB1C3, <span style="font-style:italic;">E. coli</span> DH5α didn't glow even after 24 hours incubation.<br /> | In our test, when this part was ligated into pSB1C3, <span style="font-style:italic;">E. coli</span> DH5α didn't glow even after 24 hours incubation.<br /> | ||
− | [[Image:BBa K649301 graph(2).png|thumb|center|500px| | + | [[Image:BBa K649301 graph(2).png|thumb|center|500px|Urea concentration in growth media 1 hour after IPTG induction.<br /> |
This work is done by Natsuki Kubo.]] | This work is done by Natsuki Kubo.]] | ||
+ | |||
+ | Introduction of BBa_K649301 containing arginase coding gene (<span style="font-style:italic;">rocF</span>) led to production of some urea in strain MG1655 (<span style="font-style:italic;">argR</span> +). This part produced urea more efficiently in strain JD24293 (<span style="font-style:italic;">argR</span> -). | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 08:40, 4 October 2011
Ptrc-RBS-rocF
Arginase is coded with Ptrc and RBS in this part.
rocF is the same as BBa_K649300.
Ptrc and RBS are from pTrc99A.
You should NOT ligate this part into high-copy vector.
In our test, when this part was ligated into pSB1C3, E. coli DH5α didn't glow even after 24 hours incubation.
Introduction of BBa_K649301 containing arginase coding gene (rocF) led to production of some urea in strain MG1655 (argR +). This part produced urea more efficiently in strain JD24293 (argR -).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 230
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]