Difference between revisions of "Part:BBa K631037"

Revision as of 08:19, 4 October 2011

GPC-1:eCFP:UNC-54 (for use in C. elegans)

�This construct is a C. elegans promoter (GPC-1, BBa_K631003, targets sensory neurons such as AWB) attached to a cyan fluorescent protein reporter (eCFP, BBa_K309020) and a robust C. elegans terminator (UNC-54, BBa_K309012).

An image captured using a confocal microscope is shown below. Microinjection was used to have plasmid expressed in the worm.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 2281
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2814
Illegal SapI site found at 1764
Illegal SapI.rc site found at 1596

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K631037 short</partinfo>
+�This construct is a C. elegans promoter (GPC-1, BBa_K631003, targets sensory neurons such as AWB) attached to a cyan fluorescent protein reporter (eCFP, BBa_K309020) and a robust C. elegans terminator (UNC-54, BBa_K309012).
+An image captured using a confocal microscope is shown below. Microinjection was used to have plasmid expressed in the worm.
 [[Image:Queens_Canada_eCFP_GPC-1.jpg]]