Difference between revisions of "Part:BBa K649201"
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lox2272 is a mutant of loxP which have mutations in the 8 bp spacer region. DNA segment flanked by two lox2272 marks the point which the enzyme Cre will excise. The Cre-mediated recombination of this BioBrick had been studied and proved to be working. | lox2272 is a mutant of loxP which have mutations in the 8 bp spacer region. DNA segment flanked by two lox2272 marks the point which the enzyme Cre will excise. The Cre-mediated recombination of this BioBrick had been studied and proved to be working. | ||
− | [[Image:111001DK_0.5_2272.png|thumb|center|500px|Effect of | + | [[Image:111001DK_0.5_2272.png|thumb|center|500px|Effect of Cre-meditated recombination at lox2272 cassette. ]] |
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Revision as of 04:25, 4 October 2011
PlacIQ-lox2272-rfp-lox2272-gfp
lox2272 is a mutant of loxP which have mutations in the 8 bp spacer region. DNA segment flanked by two lox2272 marks the point which the enzyme Cre will excise. The Cre-mediated recombination of this BioBrick had been studied and proved to be working.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1016
Illegal BamHI site found at 14 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 686
Illegal AgeI site found at 798 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1700