Difference between revisions of "Part:BBa K649207:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | BBa_K649207 is composed of lox2272(BBa_K649204) and PlacIQ-gfp(BBa_J54202). BBa_J54202 contains ''Bgl'' II and ''Mlu'' I restriction site on upstream of gfp. These restriction sites allows insertion of the lox227,designed to be flanked by the sticky ends of ''BamH'' I and ''Mlu'' I. This lox2272 was inserted to the PlacIQ-GFP digested with ''Bgl'' II and ''Mlu'' I. | |
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===Source=== | ===Source=== | ||
Revision as of 23:15, 3 October 2011
PlacIQ-lox2272-gfp
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 107
Illegal BamHI site found at 13 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 790
Design Notes
BBa_K649207 is composed of lox2272(BBa_K649204) and PlacIQ-gfp(BBa_J54202). BBa_J54202 contains Bgl II and Mlu I restriction site on upstream of gfp. These restriction sites allows insertion of the lox227,designed to be flanked by the sticky ends of BamH I and Mlu I. This lox2272 was inserted to the PlacIQ-GFP digested with Bgl II and Mlu I.