Difference between revisions of "Part:BBa K524004"

 
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<partinfo>BBa_K524004 short</partinfo>
 
<partinfo>BBa_K524004 short</partinfo>
  
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ori-gamma is part of the replication origin of the R6K origin. Initiation of replication at ori-gamma requires the pi protein in trans, which is encoded by the pir gene. Yet doubling the concentration of pi protein would effectively shut down the replication as well. Expression of pi protein is auto-regulated at its promoter region. Our construct design was to insert a CAP-binding site between the auto-regulating promoter and the CDS, by doing so we can halt replication and induce plasmid loss in nutrient (glucose) rich medium.
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The pir gene codes for the pi protein. Initiation of replication at ori-gamma requires the pi protein in trans. Doubling the concentration of pi protein would, however, effectively shut down the replication. Expression of pi protein is autogenously regulated.  
  
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===Usage and Biology===
 
===Usage and Biology===
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The presence of pir gene can allow maintenance of plasmids with R6K origins as their sole origin of replication.
  
 
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Revision as of 19:56, 3 October 2011

pir gene

The pir gene codes for the pi protein. Initiation of replication at ori-gamma requires the pi protein in trans. Doubling the concentration of pi protein would, however, effectively shut down the replication. Expression of pi protein is autogenously regulated.

Usage and Biology

The presence of pir gene can allow maintenance of plasmids with R6K origins as their sole origin of replication.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 438
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]