Difference between revisions of "Part:BBa K524001"
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Split sfGFPs at amino acid residues 214 and 215 have been reported to undergo spontaneous complementation to give green fluorescence.
 
Split sfGFPs at amino acid residues 214 and 215 have been reported to undergo spontaneous complementation to give green fluorescence.
  
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===Usage and Biology===
 
===Usage and Biology===
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This product of this biobrick should complement with the product of biobrick sfGFP11 [https://parts.igem.org/Part:BBa_K524002 BBa_K524002] to give green fluorescence under UV.
  
 
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Revision as of 19:40, 3 October 2011

pLac + RBS + split sfGFP 1-10

The split sfGFP1-10 was constructed from an pBAD driven sfGFP BBa_I746908. CDS of sfGFP includes amino acid residues 1-214 of sfGFP. Split sfGFPs at amino acid residues 214 and 215 have been reported to undergo spontaneous complementation to give green fluorescence.


Usage and Biology

This product of this biobrick should complement with the product of biobrick sfGFP11 BBa_K524002 to give green fluorescence under UV.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 238