Difference between revisions of "Part:BBa K518004:Experience"

Revision as of 18:51, 3 October 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K518004

User Reviews

UNIQ05cc128c06041877-partinfo-00000000-QINU

UT-Tokyo 2011

We have worked hard to demonstrate that this part enables bacteria to produce L-aspartate, but failed to do that. In the previous works (1), the amount of L-Asp was determined by HPLC. We, however, were unable to use this machinery, so we tried to detect it through alternative method, ninhydrin assay and ultraviolet-visible spectroscopy. For experimental details, see [http://2011.igem.org/Team:UT-Tokyo |our result page].

UNIQ05cc128c06041877-partinfo-00000002-QINU

@@ Line 14: / Line 14: @@
 <I>UT-Tokyo 2011</I>
 |width='60%' valign='top'|
-We have worked hard to demonstrate that this part enables bacteria to produce L-aspartate, but could not. Our sequencing results indicated a general sequence agreement, showing only a few mismatching bases. We have tried to detect the L-Asp by either ninhydrin staining or ultraviolet-visible spectroscopy, only to fail. Ninhydrin reactedwith not only amino acid (Asp) but indeed ammonium ion itself, and U-V spectroscopy detected large fumarate noise. We also tried to directly measure over-expressed intracellular AspA, but had few days to do this work.
+We have worked hard to demonstrate that this part enables bacteria to produce L-aspartate, but failed to do that. In the previous works (1), the amount of L-Asp was determined by HPLC. We, however, were unable to use this machinery, so we tried to detect it through alternative method, ninhydrin assay and ultraviolet-visible spectroscopy. For experimental details, see [http://2011.igem.org/Team:UT-Tokyo |our result page].
 <!-- Template for a user review