Difference between revisions of "Part:BBa K649301"
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You should NOT ligate this part into high-copy vector.<br />
 
You should NOT ligate this part into high-copy vector.<br />
 
In our test, when this part was ligated into pSB1C3, <span style="font-style:italic;">E. coli</span> DH5&alpha; didn't glow even after 24 hours incubation.<br />
 
In our test, when this part was ligated into pSB1C3, <span style="font-style:italic;">E. coli</span> DH5&alpha; didn't glow even after 24 hours incubation.<br />
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 +
[[Image:BBa K649301 graph.png|thumb|center|500px|introduction of <span style="font-style:italic;">rocF</span>(arginase coding gene) led to production of some urea. <br>This work was done by Natsuki Kubo and GoshiSugano.]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:32, 3 October 2011

Ptrc-RBS-rocF

Arginase is coded with Ptrc and RBS in this part.

rocF is the same as BBa_K649300.
Ptrc and RBS are from pTrc99A.

You should NOT ligate this part into high-copy vector.
In our test, when this part was ligated into pSB1C3, E. coli DH5α didn't glow even after 24 hours incubation.

introduction of rocF(arginase coding gene) led to production of some urea.
This work was done by Natsuki Kubo and GoshiSugano.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 230
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]