Difference between revisions of "Part:BBa K518004:Experience"

Revision as of 18:22, 3 October 2011

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Applications of BBa_K518004

User Reviews

UNIQbb3ec238e28493ed-partinfo-00000000-QINU

UT-Tokyo 2011

We have worked hard to demonstrate that this part enables bacteria to produce L-aspartate, but could not. Our sequencing results indicated a general sequence agreement, showing only a few mismatching bases. We have tried to detect the L-Asp by either ninhydrin staining or ultraviolet-visible spectroscopy, only to fail. Ninhydrin reactedwith not only amino acid (Asp) but indeed ammonium ion itself, and U-V spectroscopy detected large fumarate noise. We also tried to directly measure over-expressed intracellular AspA, but had few days to do this work.

UNIQbb3ec238e28493ed-partinfo-00000002-QINU

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 <I>UT-Tokyo 2011</I>
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-We have worked hard to demonstrate that this part enables bacteria to produce L-aspartate, but could not. Our sequencing results indicated a general sequence agreement, showing only a few mismatching bases. We have tried to detect the L-Asp by either ninhydrin staining or ultraviolet-visible spectroscopy, only to fail. Ninhydrin reactedwith not only amino acid (Asp) but indeed ammonium ion itself, and U-V spectroscopy detected large fumarate noise. We also tried to directly measure intracellular AspA over-expression, but have little days to this work.
+We have worked hard to demonstrate that this part enables bacteria to produce L-aspartate, but could not. Our sequencing results indicated a general sequence agreement, showing only a few mismatching bases. We have tried to detect the L-Asp by either ninhydrin staining or ultraviolet-visible spectroscopy, only to fail. Ninhydrin reactedwith not only amino acid (Asp) but indeed ammonium ion itself, and U-V spectroscopy detected large fumarate noise. We also tried to directly measure over-expressed intracellular AspA, but had few days to do this work.
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