Difference between revisions of "Part:BBa K649104"
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<partinfo>BBa_K649104 short</partinfo>
 
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[[Image:PlsrA_activity1.png|thumb|center|500px|Median fluorescence intensity(MFI) of BBa_K649104 was much higher than that of promoterless-gfp(negative control).<br>This work is done by Takuya Tsubaki.]]
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[[Image:PlsrA_activity1.png|thumb|center|700px|Median fluorescence intensity(MFI) of BBa_K649104 was much higher than that of promoterless-gfp(negative control).<br>This work is done by Takuya Tsubaki.]]
  
  

Revision as of 09:11, 3 October 2011

PlsrA-RBS-gfp

Median fluorescence intensity(MFI) of BBa_K649104 was much higher than that of promoterless-gfp(negative control).
This work is done by Takuya Tsubaki.


We measured the transcriptional activity of our lsrA promoter(BBa_K649100) by introducing a gfp gene downstream of the promoter. Its fluorescence intensity was much higher than that of promoterless-gfp(negative control), showing that our new lsrA promoter works. Moreover,fluorescence intensity of PlsrA(BBa_117002)-gfp was almost the same as a promoterless-gfp(negative control), showing that BBa_K117002 does not work properly.


We improved previous lsrA promoter(BBa_K117002).our assay of BBa_K117002


For more information, see our work in Tokyo_Tech 2011 wiki


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 770