Difference between revisions of "Part:BBa K526002:Experience"

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===Applications of BBa_K526002===
 
===Applications of BBa_K526002===
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===Attributions===
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We compared the efficiency of our temperature dependent ribo-switches with the previous temperature dependent ribo-switches present in the registry (BBa_K115017 and BBa_K115020). Both these bio-bricks favored expression only at 35 degree celsius. Our biobrick, however, favored expression only at temperatures as low as 30 degree celsius or even lesser.
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Previosuly, Olga ''et al'' (2011) described a temperature dependent riboswitch involved in regulating bacterial chemotaxis at high temperature (Fig 1A). They reported that the mRNA carrying the riboswitch was stable at all temperatures however, the protein expression was favored only at low temperature indicating a robustness in regulating bacterial chemotaxis (Fig 1B and Fig 1C).
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We believe that this low-temperature dependent riboswitch would be of great interest in heterologous protein expression. Favoring expression only at a low temperature would gurantee that the heterologous proteins are folded properly without the formation of inclusion bodies.
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In 2011 iGEM, we tried to engineer low temperature sensor into ''E. coli''. As is shown in the above figure, when GFP was fused with a temperature dependent riboswitch (Fig B) it could sense low temperature:  There was no GFP expression at 37 degree celsius. But a significant GFP expression was found at 30 degree celsius indicating the functionality of the ribo-switch.
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===User Reviews===
 
===User Reviews===

Latest revision as of 06:39, 3 October 2011

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Applications of BBa_K526002

Attributions

We compared the efficiency of our temperature dependent ribo-switches with the previous temperature dependent ribo-switches present in the registry (BBa_K115017 and BBa_K115020). Both these bio-bricks favored expression only at 35 degree celsius. Our biobrick, however, favored expression only at temperatures as low as 30 degree celsius or even lesser.

User Reviews

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