Difference between revisions of "Part:BBa K598005:Design"

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===Design Notes===
 
===Design Notes===
  
−
Obtain sequence of this RNA controller from original paper.
+
This part is designed to up-regulate downstream gene expression in the presence of theophylline.In order to obtain this part for further utilization,get it by PCR, rather than enzyme digestion.
−
Primer design.
+
−
Insert RNA controller sequence into backbone via PCR and bluntend ligation.
+
  
 
===Source===
 
===Source===
  
−
Sequence is based on original design of Beatrix Suess. Insert RNA controller sequence into backbone via PCR and bluntend ligation.
+
Sequence is based on original design of Beatrix Suess. Insert RNA controller sequence into backbone pSB1C3 via PCR and bluntend ligation.
  
 
===References===
 
===References===
 
Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004).A theophylline responsive riboswitch based on helix slipping controls gene expression ''in vivo''. Nucleic Acids Research ''32'', 1610-1614.
 
Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004).A theophylline responsive riboswitch based on helix slipping controls gene expression ''in vivo''. Nucleic Acids Research ''32'', 1610-1614.

Latest revision as of 06:32, 3 October 2011

Theophylline Responsive Riboswitch P1G1 with Native RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is designed to up-regulate downstream gene expression in the presence of theophylline.In order to obtain this part for further utilization,get it by PCR, rather than enzyme digestion.

Source

Sequence is based on original design of Beatrix Suess. Insert RNA controller sequence into backbone pSB1C3 via PCR and bluntend ligation.

References

Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004).A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research 32, 1610-1614.