Difference between revisions of "Part:BBa K598006:Design"
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| − | + | Based on original design of Beatrix Suess, we change the RBS to a more robust one. Insert RNA controller sequence into backbone pSB1C3 via PCR and bluntend ligation. | |
===References=== | ===References=== | ||
Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004).A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research 32, 1610-1614. | Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004).A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research 32, 1610-1614. | ||
Latest revision as of 06:25, 3 October 2011
Theophylline Responsive Riboswitch 1G1 with Engineered RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 71
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is designed to up-regulate downstream gene expression in the presence of theophylline. In order to obtain this part for further utilization,get it by PCR, rather than enzyme digestion.
Source
Based on original design of Beatrix Suess, we change the RBS to a more robust one. Insert RNA controller sequence into backbone pSB1C3 via PCR and bluntend ligation.
References
Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004).A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research 32, 1610-1614.