Difference between revisions of "Part:BBa K649202:Design"
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===Source=== | ===Source=== | ||
| − | + | The forward lox2272 is annealed oligonucleotide and the following lox2272 was produced by PCR (template BBa_J04450). The information of lox2272 sequence was gain from the reference below. | |
===References=== | ===References=== | ||
Site-directed integration of the cre gene mediated by Cre recombinase using a combination of mutant lox sites, Kimi Araki et al, Nucleic Acids Research 2002. | Site-directed integration of the cre gene mediated by Cre recombinase using a combination of mutant lox sites, Kimi Araki et al, Nucleic Acids Research 2002. | ||
Revision as of 14:09, 2 October 2011
PlacIQ-lox71-rfp-lox66-gfp
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1016
Illegal BamHI site found at 14 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 686
Illegal AgeI site found at 798 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1700
Design Notes
PlacIQ + lox2272 + RBS + mRFP + T7 terminator + lox2272 + RBS + GFP + T7
Source
The forward lox2272 is annealed oligonucleotide and the following lox2272 was produced by PCR (template BBa_J04450). The information of lox2272 sequence was gain from the reference below.
References
Site-directed integration of the cre gene mediated by Cre recombinase using a combination of mutant lox sites, Kimi Araki et al, Nucleic Acids Research 2002.