Difference between revisions of "Part:BBa K557000:Design"

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===Design Notes===
 
===Design Notes===
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We get it from the genome of Top10 by PCR.  
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We obtained the sequence of CheZ of Top10 in NCBI (NP416395.1) and designed primers to acquire CheZ by PCR.
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<p>forward primer:5'-GTTTCGAATTCGCGGCCGCTTCTAGATGCAACCATCAATCAAACC-3'</p>
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<p>reverse primer:5'-GTTTCCTGCAGCGGCCGCTACTAGTATTATTAAAATCCAAGTCTATCCAACAAATCGT-3'</p>
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===Source===
 
===Source===

Latest revision as of 13:21, 2 October 2011

CheZ: phosphatase which dephosphorylates CheY-P and thus plays an important role in E.Coli chemotaxi


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We obtained the sequence of CheZ of Top10 in NCBI (NP416395.1) and designed primers to acquire CheZ by PCR.


forward primer:5'-GTTTCGAATTCGCGGCCGCTTCTAGATGCAACCATCAATCAAACC-3'

reverse primer:5'-GTTTCCTGCAGCGGCCGCTACTAGTATTATTAAAATCCAAGTCTATCCAACAAATCGT-3'

Source

Escherichia coli, the strain has always been kept in the lab of USTC-IGEM

References

Shana Topp, Justin P. Gallivan (2006) Guiding Bacteria with Small Molecules and RNA. J.Am.Chem.Soc, 129,6870-6811