Difference between revisions of "Part:BBa K649105"
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<partinfo>BBa_K649105 short</partinfo>
 
<partinfo>BBa_K649105 short</partinfo>
 
      
 
      
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[[Image:OD lsrR repression.png|thumb|center|400px|After four hours from OD590 reaching 0.15, we measured OD.<br>This work is done by Hiroki Yoshise.]]
 
  
 
[[Image:LsrR repression.png|thumb|center|300px|Median fluorescence intensity(MFI) is decreased by LsrR repression.<br>This work is done by Hiroki Yoshise.]]
 
[[Image:LsrR repression.png|thumb|center|300px|Median fluorescence intensity(MFI) is decreased by LsrR repression.<br>This work is done by Hiroki Yoshise.]]

Revision as of 11:51, 2 October 2011

PlsrA-gfp-PlsrR-lsrR


Median fluorescence intensity(MFI) is decreased by LsrR repression.
This work is done by Hiroki Yoshise.


We confirmed that LsrR represses lsrA promoter. We measured LsrR repression activity by using a plasmid (BBa_K649105) which have a LsrR gene downstream of PlsrA-gfp.By the LsrR repression, the fluorescence intensity decreased 3-fold. (Fig.6). This result shows that LsrR successfully repressed PlsrA. The working parts we created allow the use of AI-2 as a signaling molecule, which is a very powerful tool to build complex Synthetic Biology systems.


For more information, see our work in Tokyo_Tech 2011 wiki


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2579
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2253
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 770