Difference between revisions of "Part:BBa K649105"
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K649105 short</partinfo>
 
<partinfo>BBa_K649105 short</partinfo>
−
 
+
   
−
Median fluorescence intensity(MFI) of BBa_K649104(PlsrA-RBS-gfp) is 3-fold higher than that of BBa_K649105(PlsrA-gfp-PlsrR-lsrR). 
+
−
+
 
[[Image:OD lsrR repression.png|thumb|center|400px|After four hours from OD590 reaching 0.15, we measured OD.<br>This work is done by Hiroki Yoshise.]]
 
[[Image:OD lsrR repression.png|thumb|center|400px|After four hours from OD590 reaching 0.15, we measured OD.<br>This work is done by Hiroki Yoshise.]]
  
Line 9: Line 7:
  
  
−
We confirmed that LsrR represses lsrA promoter.  
+
We confirmed that LsrR represses lsrA promoter. We measured LsrR repression activity by using a plasmid (BBa_K649105) which have a LsrR gene downstream of PlsrA-gfp.By the LsrR repression, the fluorescence intensity decreased 3-fold. (Fig.6). This result shows that LsrR successfully repressed PlsrA. The working parts we created allow the use of AI-2 as a signaling molecule, which is a very powerful tool to build complex Synthetic Biology systems.
  
−
For this purpose, we constructed BBa_K649105(PlsrA-gfp-PlsrR-lsrR) and compared fluorescence intensity levels of BBa_K649104(PlsrA-RBS-gfp) and BBa_K649105.
 
  
 
For more information, see our work in Tokyo_Tech 2011 wiki  
 
For more information, see our work in Tokyo_Tech 2011 wiki  

Revision as of 11:48, 2 October 2011

PlsrA-gfp-PlsrR-lsrR

After four hours from OD590 reaching 0.15, we measured OD.
This work is done by Hiroki Yoshise.
Median fluorescence intensity(MFI) is decreased by LsrR repression.
This work is done by Hiroki Yoshise.


We confirmed that LsrR represses lsrA promoter. We measured LsrR repression activity by using a plasmid (BBa_K649105) which have a LsrR gene downstream of PlsrA-gfp.By the LsrR repression, the fluorescence intensity decreased 3-fold. (Fig.6). This result shows that LsrR successfully repressed PlsrA. The working parts we created allow the use of AI-2 as a signaling molecule, which is a very powerful tool to build complex Synthetic Biology systems.


For more information, see our work in Tokyo_Tech 2011 wiki


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2579
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2253
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 770