Difference between revisions of "Part:BBa K649105"

Revision as of 09:31, 2 October 2011

PlsrA-gfp-PlsrR-lsrR

Median fluorescence intensity(MFI) of BBa_K649104(PlsrA-RBS-gfp) is 3-fold higher than that of BBa_K649105(PlsrA-gfp-PlsrR-lsrR).

After four hours from OD590 reaching 0.15, we measured OD.
This work is done by Hiroki Yoshise.

Median fluorescence intensity(MFI) is decreased by LsrR repression.
This work is done by Hiroki Yoshise.

We confirmed that LsrR represses lsrA promoter.

For this purpose, we constructed BBa_K649105(PlsrA-gfp-PlsrR-lsrR) and compared fluorescence intensity levels of BBa_K649104(PlsrA-RBS-gfp) and BBa_K649105.

For more information, see our work in Tokyo_Tech 2011 wiki

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 2579
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2253
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 770

@@ Line 4: / Line 4: @@
 Median fluorescence intensity(MFI) of BBa_K649104(PlsrA-RBS-gfp) is 3-fold higher than that of BBa_K649105(PlsrA-gfp-PlsrR-lsrR).
+[[Image:OD lsrR repression.png|thumb|center|400px|After four hours from OD590 reaching 0.15, we measured OD.<br>This work is done by Hiroki Yoshise.]]
 [[Image:LsrR repression.png|thumb|center|300px|Median fluorescence intensity(MFI) is decreased by LsrR repression.<br>This work is done by Hiroki Yoshise.]]