Difference between revisions of "Part:BBa K676006:Design"

 
 
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<partinfo>BBa_K676006 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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sdm
 
  
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The exonuclease is coded for by the D15 gene in the T5 bacteriophage genome. The gene was cloned out by PCR using designed primers. After cloning, site-directed mutagenesis was carried out to remove the XbaI restriction site loacted at the 225th to 230th nucleotide in the DNA sequence.
  
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[[Image:Primer_Design_for_Exonuclease.jpg]]
  
 
===Source===
 
===Source===
  
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T5 Bacteriophage
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T5 bacteriophage genome - 1145 to 2020 bp
  
 
===References===
 
===References===
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1. Kaliman, A.V., et al., Cloning and DNA sequence of the 5'-exonuclease gene of bacteriophage T5. FEBS Lett, 1986. 195(1-2): p. 61-4.
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2. Wang, J., et al., Complete genome sequence of bacteriophage T5. Virology, 2005. 332(1): p. 45-65.

Latest revision as of 03:23, 2 October 2011

T5 Phage Exonuclease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 600
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The exonuclease is coded for by the D15 gene in the T5 bacteriophage genome. The gene was cloned out by PCR using designed primers. After cloning, site-directed mutagenesis was carried out to remove the XbaI restriction site loacted at the 225th to 230th nucleotide in the DNA sequence.

Primer Design for Exonuclease.jpg

Source

T5 bacteriophage genome - 1145 to 2020 bp

References

1. Kaliman, A.V., et al., Cloning and DNA sequence of the 5'-exonuclease gene of bacteriophage T5. FEBS Lett, 1986. 195(1-2): p. 61-4.

2. Wang, J., et al., Complete genome sequence of bacteriophage T5. Virology, 2005. 332(1): p. 45-65.