Difference between revisions of "Part:BBa K676003:Design"
 
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===Design Notes===
 
===Design Notes===
  
−
Primers designed for PCR:
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[[Image:Primer_Design_for_TyrT_promoter.jpg]]
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−
Forward: TAGTTCGAATTCTCTAGACAGTTCATTTTTCTCAACG
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−
 
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−
Reverse: ATCATCCTGCAGACTAGTACGGGGTAATGCTTTTTAC
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−
 
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Final PCR product is 115 bp
+
  
 
===Source===
 
===Source===
  
−
Escherichia Coli K-12 genome - from 1286864 to 1286948 bp
+
Escherichia Coli K-12 genome - from 1286846 to 1286948 bp
  
 
===References===
 
===References===
 +
 +
[http://ecocyc.org/ECOLI/NEW-IMAGE?type=OPERON&object=TU00511]
  
 
[EcoCyc11] Keseler, I.M., Collado-Vides, J., Santos-Zavaleta, A., Peralta-Gil, M., Gama-Castro, S., Muniz-Rascado, L., Bonavides-Martinez, C., Paley, S., Krummenacker, M., Altman, T., Kaipa, P., Spaulding, A., Pacheco, J., Latendresse, M., Fulcher, C., Sarker, M., Shearer, A.G., Mackie, A., Paulsen, I., Gunsalus, R.P., and Karp, P.D.,
 
[EcoCyc11] Keseler, I.M., Collado-Vides, J., Santos-Zavaleta, A., Peralta-Gil, M., Gama-Castro, S., Muniz-Rascado, L., Bonavides-Martinez, C., Paley, S., Krummenacker, M., Altman, T., Kaipa, P., Spaulding, A., Pacheco, J., Latendresse, M., Fulcher, C., Sarker, M., Shearer, A.G., Mackie, A., Paulsen, I., Gunsalus, R.P., and Karp, P.D.,
 
EcoCyc: a comprehensive database of Escherichia coli biology
 
EcoCyc: a comprehensive database of Escherichia coli biology
 
Nucleic Acids Research 39:D583-590 2011.
 
Nucleic Acids Research 39:D583-590 2011.

Latest revision as of 23:13, 1 October 2011

TyrT Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primer Design for TyrT promoter.jpg

Source

Escherichia Coli K-12 genome - from 1286846 to 1286948 bp

References

[http://ecocyc.org/ECOLI/NEW-IMAGE?type=OPERON&object=TU00511]

[EcoCyc11] Keseler, I.M., Collado-Vides, J., Santos-Zavaleta, A., Peralta-Gil, M., Gama-Castro, S., Muniz-Rascado, L., Bonavides-Martinez, C., Paley, S., Krummenacker, M., Altman, T., Kaipa, P., Spaulding, A., Pacheco, J., Latendresse, M., Fulcher, C., Sarker, M., Shearer, A.G., Mackie, A., Paulsen, I., Gunsalus, R.P., and Karp, P.D., EcoCyc: a comprehensive database of Escherichia coli biology Nucleic Acids Research 39:D583-590 2011.