Difference between revisions of "Part:BBa K648034"
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| + | We used this part to test that the promoter on the right side of Or was working properly. The production of red fluorescence protein was measured using a TECAN machine, and compared to a normal strand of E. coli. The data collected while the samples were in exponential growth was used to determine a rate of expression of the rfp. Compared to the control, the rate of fluorescence was much higher in our test subject, about 4227 compared to 418 (fu/OD600nm). This suggests that the promoter on Or is functional. A graph of the results is shown below. | ||
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| + | [[image:PSUOrTest.png|800px]] | ||
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| + | [[Image:OrTest2.jpg|800px]] | ||
Latest revision as of 21:54, 1 October 2011
Or tester
This part consists of the operating region (Or) for the lambda phage, and also a ribosome binding sight, mcherry with an rfp and a stop codon.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
We used this part to test that the promoter on the right side of Or was working properly. The production of red fluorescence protein was measured using a TECAN machine, and compared to a normal strand of E. coli. The data collected while the samples were in exponential growth was used to determine a rate of expression of the rfp. Compared to the control, the rate of fluorescence was much higher in our test subject, about 4227 compared to 418 (fu/OD600nm). This suggests that the promoter on Or is functional. A graph of the results is shown below.
