Difference between revisions of "Part:BBa K649001"
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Generally, in the presence of 3O-C12-HSL, lasR activates lasI promoter and the transcription level of downstream gene increases, but in the absence of 3O-C12-HSL, lasR can't activate lasI promoter. 2011 iGEM Tokyo-Tech characterized BBa_K649000 by means of using BBa_K649001 composed of BBa_K649000 and BBa_J54103.
 
Generally, in the presence of 3O-C12-HSL, lasR activates lasI promoter and the transcription level of downstream gene increases, but in the absence of 3O-C12-HSL, lasR can't activate lasI promoter. 2011 iGEM Tokyo-Tech characterized BBa_K649000 by means of using BBa_K649001 composed of BBa_K649000 and BBa_J54103.
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We improved previous lasI promoter(BBa_J64010).[https://parts.igem.org/Part:BBa_J64010:Experience our assay of BBa_J64010]
  
  

Revision as of 21:50, 1 October 2011

GFP regulated by 3OC12-HSL and LasR

Fluorescence intensity of BBa_K649001 was increased by 3O-C12-HSL induction.


Effect of 3O-C12-HSL induction on fluorescence intensity
This work is done by Takuya Tsubaki.


Generally, in the presence of 3O-C12-HSL, lasR activates lasI promoter and the transcription level of downstream gene increases, but in the absence of 3O-C12-HSL, lasR can't activate lasI promoter. 2011 iGEM Tokyo-Tech characterized BBa_K649000 by means of using BBa_K649001 composed of BBa_K649000 and BBa_J54103.


We improved previous lasI promoter(BBa_J64010).our assay of BBa_J64010


For more information, see our work in Tokyo_Tech 2011 wiki


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 118
    Illegal BamHI site found at 106
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 805