Difference between revisions of "Part:BBa K676004:Design"

 
 
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<partinfo>BBa_K676004 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
Primer and 3 way ligation
 
  
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Device constructed by performing 3A assembly between BBa_K676003 (TyrT promoter) and BBa_E0422 (RBA+eCFP+Ter) and pSB1C3 plasmid backbone.
  
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[[Image:UCL-Content-Experiment_Plan_for_Supercoilometer.jpg]]
  
 
===Source===
 
===Source===
  
E Coli promoter and iGEM kit plate CFP expression casette
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TyrT promoter from E coli genome and CFP expression casette from iGEM kit plate (Spring Distribution 2011 Kit Plate 1 - 8M).
 
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===References===
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Latest revision as of 21:07, 1 October 2011

Supercoilometer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Device constructed by performing 3A assembly between BBa_K676003 (TyrT promoter) and BBa_E0422 (RBA+eCFP+Ter) and pSB1C3 plasmid backbone.

UCL-Content-Experiment Plan for Supercoilometer.jpg

Source

TyrT promoter from E coli genome and CFP expression casette from iGEM kit plate (Spring Distribution 2011 Kit Plate 1 - 8M).