Difference between revisions of "Part:pSB6A1:Experience"

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This minimization yielded a reduction in size from 4022 bp to 2743 bp.
 
This minimization yielded a reduction in size from 4022 bp to 2743 bp.
  
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For comparison of the copy number of the plasmids, triplicates of OD normalized bacterial cultures containing the respective plasmids were miniprepped and the DNA concentration determined by an agarose DNA gel of a dilution series of an EcoRI digest.
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For comparison of the copy number of the plasmids, triplicates of OD normalized bacterial cultures containing the respective plasmids were miniprepped. The DNA concentration was then determined by an agarose DNA gel electrophoresis of EcoRI digest of the purified plasmids.
 
This experiment showed a clear reduction of  
 
This experiment showed a clear reduction of  
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[[Image:Veeeeeeery final copy number test gel.png|400px]]
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[[Image:Veeeeeeery final copy number test gel.png|100px|left|thumb|'''Figure 1: Agarose gel''' showing the comparison of the .]]
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Revision as of 15:55, 1 October 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of pSB6A1

User Reviews

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This plasmid has been adapted to the version 5 of BioBrick vector backbones by the ETHZ 2011 iGEM team. This means, that the cassettes for the ORI and antibiotic resistance have been minimized (reduction of the size and transcriptional terminators flanking the prefix and the suffix have been added, to trancriptionally insulate the inserted parts from the vector backbone machinery and vice versa. This minimization yielded a reduction in size from 4022 bp to 2743 bp.

For comparison of the copy number of the plasmids, triplicates of OD normalized bacterial cultures containing the respective plasmids were miniprepped. The DNA concentration was then determined by an agarose DNA gel electrophoresis of EcoRI digest of the purified plasmids. This experiment showed a clear reduction of

Figure 1: Agarose gel showing the comparison of the .


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