Difference between revisions of "Part:BBa K514000"

Revision as of 12:15, 1 October 2011

C6-3OXO-HSL -> β-galactosidase activity

This BioBrick is a composition of F2620 with I732019. It was conceived to characterize the construction F2620: check which concentration of its specific quorum sensing signal (C6-3-oxo-HSL) it's able to detect, and how long does it take the bacterium to produce an answer (in this case, the expression of lacZ).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1004

Characterization

We tested this construction by two different methods. Firstly, we seeded the transformed bacteria on a plate with 80ul of X-Gal 1% and 1 ul of C6-3-oxo-HSL 10mg/ml, just to check that it worked. For the proper charaterization we resorted to an ONPG essay. Ortho-nitrophenyl-betha-galactoside is an artificial substrate that turns the liquid media yellow when it's hydrolized by the betha-galactosidase. We measured

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 <partinfo>BBa_K514000 short</partinfo>
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 <partinfo>BBa_K514000 SequenceAndFeatures</partinfo>
+===Characterization===
+We tested this construction by two different methods.
+Firstly, we seeded the transformed bacteria on a plate with 80ul of X-Gal 1% and 1 ul of C6-3-oxo-HSL 10mg/ml, just to check that it worked.
+For the proper charaterization we resorted to an ONPG essay. Ortho-nitrophenyl-betha-galactoside is an artificial substrate that turns the liquid media yellow when it's hydrolized by the betha-galactosidase.
+We measured
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